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KMID : 0371320040660020073
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Journal of the Korean Surgical Society
2004 Volume.66 No. 2 p.73 ~ p.80
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Expression of Factor Ⅷ Related Antigen and Changes of Serum IL-1α, IL-1β and IL-6 during Diethylnitrosamine (DEN) Induced Carcinogenesis in Rat Liver
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Park Yong-Gum
Lee Hye-Sun
Park Eon-Sub
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Abstract
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Purpose: In order to elucidate the tumor angiogenesis and to evaluate the usefulness of some cytokines as parameters of tumor progression and metastasis, we investigated the expression of Factor Ⅷ related antigen (Factor Ⅷ R-Ag) and measured the plasma levels of interleukins -1α(IL-1α), 1β (IL-1β) and 6 (IL-6) during rat liver tumorigenesis.
Methods: Sorague-Dawley rats were given diethylnitrosamine (DEN) by water, and sequentially sacrificed. For evaluation we performed light microscopy, immunohistochemical study using avidin-biotin complex, and ELISA.
Results: During the carcinogenesis, the staining, of Factor Ⅷ R-Ag was negative in normal livers, focal in hyperplastic nodules, scattered in hepatocellular adenomas and diffusely positive in hepatocellular carcinomas. The serum level of IL-1α peaked at 9 weeks, that of IL-1β steadily increased to peak at 12 weeks, and that of IL-6 continuously increased after DEN a administration. In addition, the serum concentration of IL-6 was markedly elevated in hepatocellular carcinomas metastasized to the lung.
Conclusion: From the above results it could be suggested that sinusoical phenotypic acquisition of Factor Ⅷ R-Ag is linked to the development of hepatocellular carcinomas and that serum levels of IL-1β and IL-6 are related to the multistep development of hepatocellular carcinoma. Furthermore, excess production of IL-6 may be a useful parameter to predict metastatic potentials.
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